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Lysates were sonicated for 20 s (25% power, 0.5 cycles), centrifuged at 12,000 xg for 30 min at 4 C, and the clarified supernatant was transferred to a new tube

We included original clinical research, meta-analyses, and major guidelines that examined the association between genetic variants in these genes and antidepressant pharmacokinetics, efficacy, or tolerability in adults or pediatric populations

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This therapy involves implanting electrodes connected to a pulse generator to deliver electrical signals to specific brain targets, thereby modulating neural circuits [197, 198]