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cells harvested at OD 600 0.6-0.8 were washed twice and resuspended in sterile PBS to 1 10 9 CFU/mL (titer verified by anaerobic plate counts with serial dilution)

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(70) reported that the administration of organic trace elements (Zn, Mn, Cu and Co) to periparturient dairy cows promotes the innate immune response of their newborn calves by reducing the expression of inflammatory mediators in blood neutrophils

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