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Quantitative sequence detection was carried out on an ABI PRISM 7700 by adding to the reverse transcribed reaction 2.5 M forward and reverse primers, 500 M of each dNTP, buffer and 5U AmpliTaq Gold

In our inactive conformations, F397 is stacked between the nicotinamide and the isoalloxazine rings (Fig

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The selection of transformants was also conducted in 0.5 MS-agar plates without sucrose and supplemented with 50 g/ml cefotaxime, 100 g /ml kanamycin, and followed up to the T3 generation to obtain homozygous lines

(4) at least a $2,000 difference in average costs between the MCC subgroup and the with CC subgroup and between the CC subgroup and NonCC subgroup

Crit Rev Toxicol 36(4):359385