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The concern is practical rather than safety-based: receptor desensitization, diminishing returns, and cost

The plasmids used in this study were shown as follows: pcDNA3.13 Flag, pcDNA3.1-rNdufa1-3 Flag-C, pcDNA3.1-rCREB1-3 Flag-C, pDualuc-Basic, Ndufa1-pDualuc-Basic were from UNIBIO Biological Technology (Changsha, China)