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Incubate cells at 4 C for 10 min followed by centrifugation for 10 min at 14,000 rpm and 4 C

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Serious side effects are rare at standard oral doses but possible with high-dose intravenous infusions, particularly allergic reactions or phlebitis

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2.5.2 LCMS/MS data-dependent acquisition Digested samples were analyzed by nano HPLC using an EASY-nLC 1,000 chromatograph coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, Pittsburgh, PA, United States), using an EASY-Spray Accucore (P/N ES801) Thermo Scientific reverse phase column coupled to an EASY-SPRAY Electro Spray ionizer (Thermo Fisher Scientific, Pittsburgh, PA, United States), with Spray voltage between 2.53.5 kV

Some limitations of antibodies for clinical use are slow diffusion into tumor tissue, limited in vivo stability, and high production costs [7, 11, 12]