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MS-based proteomics Hypothalamic tissue was powdered and lysed (lysis buffer 50 mM Tris, 4% SDS buffer, pH 8.5) using BeatBox homogenizer (PreOmics)

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Br J Cancer 117(4):563571 Tominaga N, Kosaka N, Ono M, Katsuda T, Yoshioka Y, Tamura K et al (2015) Brain metastatic cancer cells release microRNA-181c-containing extracellular vesicles capable of destructing blood-brain barrier