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To identify specific interactors, even those in low abundance while eliminating abundant contaminant proteins, cell lines stably expressing the LAP-tag-containing wild-type FUS/TLS were grown in isotopically heavy medium containing 13 C 6 , 15 N 4 -arginine and 13 C 6 , 15 N 2 -lysine, while the parental line (that is, no transgene) was grown in light medium containing normal arginine and lysine (Fig